Developmentally regulated alternate 3’ end cleavage of nascent transcripts controls dynamic changes in protein expression in an adult stem cell lineage

To determine the extent of alternative 3' end cleavage during Drosophila spermatogenesis, we performed 3' end sequencing of Drosophila testes from flies at different time points post heat shock (pHS) that were mutant for bam and also had a heat shock inducible transgene expressing Bam. We then performed Polysome profiling of testes at 24, 48 and 72 hours PHS and created 3' end sequencing libraries from the free, 40S, 60s, 80S, 2-3 ribosomes, and 4+ ribosomes fractions for each dataset 3' end sequencing of testes from flies (genotype: bam-/-; hsBam) 0, 16, 24, 32, 48, 72 hours PHS and 3' end sequencing of Polysome Fractions (free, 40S, 60S, 80S, 2-3ribosomes, 4+ ribosomes) of testes from flies 24, 48 and 72 hours PHS