Source code 2: Test datasets for OPT reconstruction and registration (part 3)

These RAR archive files contain the following: (1) acquisition data from our OPT platform of a 2 dpf fezf2 mutant embryo stained with an in situ probe for ascl1a (folder 'data\OPT', extension '.mat'). This file serves as a test dataset for our OPT reconstruction source code. (2) Unstained reference fish (URFs; folder 'data\Registration\ referenceFish') and OPT reconstructions from 8 wild-type embryos (folder 'data\Registration\TestData_th_2dpf\wt') and 8 fezf2 mutant embryos (folder 'data\Registration\TestData_th_2dpf\mt') stained with an in situ probe for tyrosine hydroxylase (th). URFs for 2 dpf and 3 dpf are provided. All th-stained embryos are 2 dpf. These images serve as a test dataset for our registration source code. Source code for both OPT reconstruction and registration is available at https://github.com/aallalou/OPT-InSitu-Toolbox. Download all RAR archive files (parts 1 through 7) prior to extraction.

本RAR压缩归档文件包含以下内容：(1) 我们的OPT（Optical Projection Tomography，光学投影层析成像）平台采集的、针对ascl1a基因使用原位杂交探针染色的2 dpf（受精后天数，days post fertilization）的fezf2突变体胚胎的采集数据，存储于文件夹'dataOPT'，文件扩展名为.mat。该数据集可作为我们的OPT重建源代码的测试数据集。(2) 无染色对照鱼（Unstained Reference Fish, URFs，存储于文件夹'dataRegistration referenceFish'），以及经酪氨酸羟化酶（tyrosine hydroxylase, th）原位杂交探针染色的8条野生型胚胎（存储于文件夹'dataRegistrationTestData_th_2dpfwt'）和8条fezf2突变体胚胎的OPT重建结果，存储于文件夹'dataRegistrationTestData_th_2dpfmt'。本数据集提供了2 dpf和3 dpf的无染色对照鱼，所有经th染色的胚胎均为2 dpf样本。上述图像可作为我们的配准源代码的测试数据集。OPT重建与配准的源代码可在https://github.com/aallalou/OPT-InSitu-Toolbox获取。请在解压前下载全部7个分卷RAR压缩归档文件。