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RNA-seq of resting and stimulated CD4+ and CD8+ T cells from wt and Raptor-deleted mice

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NIAID Data Ecosystem2026-05-02 收录
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To explore RNA expression in resting and activated T cells total RNA without rRNA depletion was analyzed. Magnetically sorted CD4+ and CD8+ T cells from wt and Raptor-deleted mice were incubated with IL-7 (unstimulated) or plate-bound CD3 and CD28 mAbs. Live cells were sorted as FVD780- and MFG-E8-negative for flow cytometry.

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2024-12-30
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