The proteomics analysis data of the endosomes from mice blood-brain-barrier under sleep deprivation and recovery VS sleep deprivation only, ZT8VS ZT20.

To investigate the content and function of the BBB endothelium are modulated by sleep, we sought to determine whether the protein profile of the endothelium is affected by sleep. One group of mice to 6 hours of sleep deprivation (SD), starting at Zeitgeber Time (ZT0) or lights on, followed by 2 hours of recovery sleep, whereas a second group mice underwent 8 hours of SD. To determine if similar changes occur with sleep in a daily cycle, we collected brain endothelial cells from mice at two time points, ZT8 (sleep phase) and ZT20 (wake phase). Endosomes isolated from these cells were subjected to proteomic analysis using LC–MS/MS.为探究睡眠如何调控血脑屏障（Blood-Brain Barrier, BBB）内皮细胞的组成与功能，本研究旨在明确内皮细胞的蛋白质谱是否受睡眠影响。实验分为两组小鼠：一组于授时因子时间0（Zeitgeber Time 0，ZT0，即灯光开启时刻）开始接受6小时睡眠剥夺（Sleep Deprivation，SD），随后进行2小时恢复睡眠；另一组小鼠则接受8小时睡眠剥夺（SD）。为验证睡眠相关变化是否存在每日节律，研究人员分别在ZT8（睡眠时段）与ZT20（觉醒时段）两个时间点从小鼠体内采集脑内皮细胞。对从上述细胞中分离得到的内体，采用液相色谱-串联质谱（Liquid Chromatography-Tandem Mass Spectrometry，LC-MS/MS）开展蛋白质组学分析。