soil metagenome Genome sequencing and assembly. soil metagenome

A study was conducted to investigate the effect of ecological restoration on soil microbial communities, with lakeside wetland changing from abandoned grassland (GL) to flower nursery land (FL), natural reed wetland (RL) and poplar plantation land (PL). Quantitative PCR and Illumina Misep sequencing of 16S rRNA genes V3-V4 region were used to estimate the genetic diversity of microorganisms among these four patterns.Our findings suggest that ecological restoration of natural recovery and artificial plantation leads to more diverse of bacterial communities and soil nutrient enrichment that decreasing some bacterial groups with strong metabolic activities in oligotrophic environment. This provides us a insight to investigate the relationship between soil nutrient cycling and microorganism community composition along the restoration process of Chaohu Lake wetland.