Additional file 2: Table S1. of High-throughput sequencing of pituitary and hypothalamic microRNA transcriptome associated with high rate of egg production

The main reproductive traits of the selected LP and HP samples. Table S2. Primer sequences for real-time PCR validation and SNP detection. Table S3. Overview of miRNA-seq data of all samples. Table S4. Identities of various RNA sequences in pituitary and hypothalamic tissues. Table S5. Expression profile of known miRNAs in pituitary and hypothalamic tissues of low- and high-rate egg production chickens. Table S6. Novel miRNAs expressed in pituitary and hypothalamus tissues of low- and high-rate egg production chickens. Table S7. The distribution of miRNA pairs in pituitary and hypothalamic tissues. Table S8. Differentially expressed miRNAs between low- and high-rate egg production chickens in pituitary and hypothalamic tissues. Table S9. The prediction of the differentially expressed miRNA targets in pituitary and hypothalamic tissues. Table S10. GO and KEGG pathway annotations for the miRNA targets in pituitary and hypothalamic tissues. Table S11. miRNAs and their predicted target genes involved in the regulation processes of reproduction. Table S12. Differentially expressed genes between low- and high-rate egg production chickens in pituitary and hypothalamic tissues. Table S13. The predicted miRNA-target pairs that are differentially and reciprocally expressed in pituitary and hypothalamic tissues. Table S14. The haplotype distribution and sequence variations in the selected miRNA precursors. (XLSX 1731Â kb)

所选低产蛋率（LP）与高产蛋率（HP）样本的主要繁殖性状。表S2：用于实时荧光定量PCR（real-time PCR）验证及单核苷酸多态性（SNP）检测的引物序列。表S3：所有样本的microRNA测序（miRNA-seq）数据概况。表S4：垂体与下丘脑组织中各类RNA序列的一致性信息。表S5：低产与高产蛋率鸡只垂体及下丘脑组织中已知microRNA的表达谱。表S6：低产与高产蛋率鸡只垂体及下丘脑组织中表达的新型microRNA。表S7：垂体与下丘脑组织中microRNA配对的分布情况。表S8：低产与高产蛋率鸡只垂体及下丘脑组织中差异表达的microRNA。表S9：垂体与下丘脑组织中差异表达microRNA的靶基因预测结果。表S10：垂体与下丘脑组织中microRNA靶基因的基因本体论（GO）及京都基因与基因组百科全书（KEGG）通路注释信息。表S11：参与繁殖调控过程的microRNA及其预测靶基因。表S12：低产与高产蛋率鸡只垂体及下丘脑组织中差异表达的基因。表S13：在垂体与下丘脑组织中呈差异且反向表达的microRNA-靶基因配对预测结果。表S14：所选microRNA前体的单倍型分布及序列变异情况。（XLSX格式，文件大小1731 KB）