ASH2L controls ureteric bud morphogenesis via regulation of RET/GFRA1 signaling activity [CUT&Tag]

To elucidate the role of H3K4 methylation in metanephros development, we selectively inactivated ASH2L, core subunit of the COMPASS complex in mice UB lineage. We then performed histone H3K4me3 modification landscape profiling of the control (Hoxb7Cre-GFP+,Ash2l F/+) and mutant (Hoxb7Cre-GFP+,Ash2l F/F) FACS-sorted UB lineage cells at E16.5 by CUT&Tag-seq. CUT&tag-seq for histoneH3K4me3 in FACS-sorted control (Hoxb7Cre-GFP+,Ash2l F/+) and mutant (Hoxb7Cre-GFP+,Ash2l F/F) FACS-sorted UB lineage cells at E16.5.