Images showing a rapid differential staining technique for Fusarium pseudograminearum in cereal tissues during crown rot infections

Images of wheat and barley tissues colonised by the pathogens Fusarium pseudograminearum, Blumeria graminis f. sp. hordei and Puccinia striiformis were obtained after staining using a novel technique. This new technique was demonstrated to allow differential staining of host and pathogen tissues in leaves and stems. Several staining combinations were assessed. The selected procedure using solophenyl flavine 7GFE and safranin provided high contrast informative images of fungal and plant structures during pathogenesis.

本数据集包含经新型染色技术处理后，被假禾谷镰孢菌（Fusarium pseudograminearum）、大麦白粉菌（Blumeria graminis f. sp. hordei）以及小麦条锈病菌（Puccinia striiformis）定殖的小麦与大麦组织图像。该新型染色技术可实现叶片与茎部组织中宿主植物与病原菌的差异化染色，这一特性已得到验证。研究中对多种染色组合方案进行了评估筛选，最终选定的以索洛苯基荧光素7GFE（solophenyl flavine 7GFE）与番红（safranin）为染料的染色流程，可在病原菌侵染过程中为真菌与植物结构提供高对比度、信息丰富的成像结果。