Seurat Object: scRNA-seq of HSCs/HPCs under 5 oxygen tensions

Single cell RNA-sequencing was performed on 4 umbilical cord blood units enriched for CD34+ cells, either expanded or unmanipulated. Two units were expanded under five different oxygen tensions (1%, 3%, 5%, 14%, and 21%) for two days, while two were expanded for seven days. For two units, we also sequenced unmanipulated (never cultured) CD34+ cells.