Ent1-mNeonGreen and Abp1-mTq TIRF Raw data from "Sla2 is a core interaction hub for Clathrin Light Chain and the Pan1/End3/Sla1 Complex"
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This dataset is raw TIRF movies (as ND2 files) of data shown in Figure 4e of the manuscript "Sla2 is a core interaction hub for Clathrin Light Chain and the Pan1/End3/Sla1 Complex" in BioXiv (https://doi.org/10.1101/2024.11.14.623549) In this repository, you will find 20 different movies taken over two microscopy sessions for Ent1-mNeonGreen/Abp1-mTurquoise2 for yeast cells expressing Sla2-WT and two different mutants (called Site1 and Site2 mutants). How is it called in the repository / In the manuscriptSla2-WT - Sla2 - WTSla2-dYYR - Sla2 - ΔSite2Sla2-d515 - Sla2 - ΔSite1 Data relating to experimental conditions can be found in https://doi.org/10.1101/2024.11.14.623549
本数据集为手稿《Sla2是网格蛋白轻链与Pan1/End3/Sla1复合物的核心互作枢纽》(发表于预印本平台BioRxiv,DOI: 10.1101/2024.11.14.623549)中图4e所展示实验数据的原始总内反射荧光显微镜(Total Internal Reflection Fluorescence, TIRF)成像电影文件,文件格式为ND2。 本存储库中共包含20组独立的显微镜成像电影,采集自两次成像实验,对应表达Sla2野生型(Sla2-WT)以及两种分别命名为Site1突变体、Site2突变体的酵母细胞中Ent1-mNeonGreen与Abp1-mTurquoise2的双标记成像结果。 本存储库与手稿中的样本命名对应关系如下: Sla2-WT → Sla2 - WT Sla2-dYYR → Sla2 - ΔSite2 Sla2-d515 → Sla2 - ΔSite1 实验相关的详细条件可参阅上述DOI链接:https://doi.org/10.1101/2024.11.14.623549。



