Urotensin II/GPR14 Pathway Regulates Chronic Itch in Mice

In order to analyze the regulatory effects of UII and GPR14 on gene transcription in Mc903-induced atopic dermatitis skin, ear skin collected from MC903 chronic itch mouse model on day 13 was used for in-depth analysis and sequencing of skin gene expression. The effect of GSK1562590 on the expression of pruritus and inflammatory factors in dermatitis was detected by RNA-seq. Overall design: Mice were evenly divided into two groups according to body weight. MC903 was applied to the left ear and anhydrous ethanol was applied to the right ear for seven consecutive days. On the 8th day after the success of the model, mc903 was applied to the left ear of mice and anhydrous ethanol was applied to the right ear, and then the mice were treated with equilibrium treatment for 1 hour. After 1 hour, the mice were treated with gavage immediately. The inhibitor group was given GSK1562590(5mg/kg, dose 100ul); The positive control group only applied the same volume of vehicle. Immediately after the drug was applied, mice were subjected to behavioral video 60mim. After the MC903 chronic pruria model was completed, mouse ear skin was extracted for RNA-seq.