Data from: Novel amphiphilic polyvinylpyrrolidone (PVP) functionalized silicone particles as carrier for low cost lipase immobilization

The high catalytic activity, specificity and stability of immobilization lipase have been drawn a great interests. How to reduce the cost of support materials has always been a hot topic in this field. Herein, for the development of low-cost immobilized lipase, we demonstrate an amphiphilic polyvinylpyrrolidone (PVP) grafted on silicone particles (SP) surface materials (SP-PVP) with rational design based on interfacial activation and solution polymerization. Meanwhile, the hydrophilic pristine SP and hydrophobic polystyrene-corded silicone particles (SP-Pst) were also prepared for lipase immobilization. The SP-PVP were characterized by X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transforms infrared spectroscopy and thermogravimetry. Our results indicated that the lipase loading amount on the SP-PVP composites was about 215 mg of protein per g. In the activity assay, the immobilized lipase SP-PVP@CRL exhibited higher catalysis activity, better thermostability and reusability than that of SP@CRL and SP-Pst@CRL. The immobilized lipase retained more than 54 % of its initial activities after 10 times reuse and approximately trended to a steady in the following cycles. By introducing the interesting amphiphilic polymer to these cheap and easily obtained silicone particles (SP) surface, the relative performance of immobilized lipase can be significantly improved, rendering interactions between the low cost supports materials and lipase.

固定化脂肪酶（immobilized lipase）凭借其优异的催化活性、特异性与稳定性，受到了广泛关注。如何降低载体材料的成本，始终是该领域的研究热点。为此，为开发低成本固定化脂肪酶，本研究基于界面活化与溶液聚合的合理设计，制备了一种接枝两亲性聚乙烯吡咯烷酮（polyvinylpyrrolidone, PVP）的硅颗粒（silicone particles, SP）表面材料（SP-PVP）。同时，本研究还制备了亲水型原始硅颗粒以及疏水型聚苯乙烯修饰硅颗粒（SP-Pst），用于脂肪酶固定化。采用X射线衍射（X-ray diffraction, XRD）、扫描电子显微镜（scanning electron microscopy, SEM）、X射线光电子能谱（X-ray photoelectron spectroscopy, XPS）、傅里叶变换红外光谱（Fourier transforms infrared spectroscopy, FTIR）以及热重分析（thermogravimetry, TG）对SP-PVP进行了表征。研究结果表明，SP-PVP复合材料的脂肪酶负载量可达约215 mg蛋白质每克载体。活性检测结果显示，SP-PVP@CRL固定化脂肪酶的催化活性、热稳定性与重复使用性能均优于SP@CRL与SP-Pst@CRL。该固定化脂肪酶在重复使用10次后，仍保留了超过54%的初始活性，且在后续循环中活性趋于稳定。通过将两亲性聚合物引入这类廉价易得的硅颗粒（SP）表面，可显著提升固定化脂肪酶的综合性能，强化低成本载体与脂肪酶之间的相互作用。