AGRICULTURAL CROP RESIDUES: ITS POTENTIAL AS NUTRIENT MEDIA IN IN-VITRO CULTURE OF OYSTER MUSHROOM (Pleurotus Ostreatus)

The disposal of agri-waste is one of the most pressing problems facing the Philippines today. Farmers have chosen to burn agricultural waste as a disposal method, which pollutes the environment and leads to climate change. A study was conducted to determine the potential of different crop residues as agar-based nutrient media for the in-vitro culture of Oyster Mushroom (Pleurotus ostreatus). The study was lay-outed using Completely Randomized Design (CRD) replicated three times with 7 treatments include Water Hyacinth Agar (WHA), 50% Banana Leaves and 50% Bracts Agar (BLBA), 50% Rice Straw and 50% Hull Agar (RSHA), Palm Oil Empty Bunch Agar (POEBA), 50% Corn Cobs and 50% Husk Agar (CCHA), Coconut Coir Agar (CCA) with Potato Dextrose Agar (PDA) as control. The different media were prepared aseptically in the laboratory, and isolation and data collection took place 7-14 days later. Results showed that crop residues from Water Hyacinth, Banana Leaves and Bracts, Rice Straw and Hull, Palm Oil Empty Bunch, and Coco Coir were found to be a good substitute media for oyster mushroom development, as the substrate promotes oyster mushroom growth and colonization in in-vitro culture. However, since it colonized the media in such a short time, Palm Oil empty bunch may be one of the best alternatives for PDA.

农业废弃物处置是当前菲律宾面临的最紧迫问题之一。农户常选择焚烧农业废弃物作为处置方式，此举不仅污染环境，还会加剧气候变化。本研究旨在探究不同作物残体作为琼脂基营养培养基，用于平菇（Pleurotus ostreatus）体外（in vitro）培养的潜力。本研究采用完全随机设计（Completely Randomized Design, CRD），设置3次生物学重复，共包含7组处理：水葫芦琼脂（Water Hyacinth Agar, WHA）、50%香蕉叶与50%苞片琼脂（50% Banana Leaves and 50% Bracts Agar, BLBA）、50%稻草与50%稻壳琼脂（50% Rice Straw and 50% Hull Agar, RSHA）、油棕空果串琼脂（Palm Oil Empty Bunch Agar, POEBA）、50%玉米芯与50%玉米苞衣琼脂（50% Corn Cobs and 50% Husk Agar, CCHA）、椰子椰糠琼脂（Coconut Coir Agar, CCA），并以马铃薯葡萄糖琼脂（Potato Dextrose Agar, PDA）作为空白对照。所有培养基均在实验室中无菌制备，后续的菌种分离与数据采集工作于接种后7-14天开展。研究结果表明，水葫芦、香蕉叶与苞片、稻草与稻壳、油棕空果串以及椰糠的作物残体均可作为平菇体外培养的优良替代培养基，该类基质可有效促进平菇在体外培养中的生长与定殖。但油棕空果串基质可在极短时间内完成培养基定殖，因此或为替代马铃薯葡萄糖琼脂的最优选择之一。