LC3B-II fluorescence intensity in PBMCs from healthy donors and ME/CFS patients (CTRL + wIRA)

Our research hypothesis was that heat treatment by water-filtered infrared-A (wIRA) irradiation at 39 °C for 60 minutes would lead to increased autophagy compared to an untreated control. The following files show the treatment of PBMCs from healthy donors and ME/CFS patients. The files show the median fluorescence intensity from healthy donors and ME/CFS patients as triplicates in untreated (CTRL) and treated (wIRA) cells. Or the mean values of the triplicates of each condition to compare healthy donors with ME/CFS patients. The data show a higher level of LC3B-II in ME/CFS patients compared to healthy donors and an increase in autophagy due to treatment with wIRA.

本研究的假设为：采用经水过滤的红外-A（water-filtered infrared-A，wIRA）辐射在39℃下处理60分钟，可使细胞自噬水平高于未处理对照组。本数据集包含健康供体与肌痛性脑脊髓炎/慢性疲劳综合征（Myalgic Encephalomyelitis/Chronic Fatigue Syndrome，ME/CFS）患者的外周血单个核细胞（peripheral blood mononuclear cell，PBMCs）的相关实验处理数据。相关文件分别展示了未处理（CTRL）与经wIRA处理的细胞中，健康供体及ME/CFS患者的中位荧光强度数据，每组均设置三次重复实验；或以各实验条件下三次重复的平均值进行统计，用于对比健康供体与ME/CFS患者的指标差异。实验数据显示，与健康供体相比，ME/CFS患者的LC3B-II蛋白水平更高，且经wIRA处理后细胞自噬水平有所提升。