Processed FACS data for "Evolutionarily conserved transcriptional regulators control monoaminergic neuron development"

Contents This dataset contains raw fluorescence-activated cell sorting (FACS) data corresponding to the following samples:QC_Setup, hrs_00_03_Rep1_A1, hrs_00_03_Rep2_A9, hrs_02_05_Rep1_A2, hrs_02_05_Rep2_A10, hrs_04_07_Rep1_A3, hrs_04_07_Rep2_A11, hrs_05_08_Rep1_A4, hrs_05_08_Rep2_A12, hrs_06_10_Rep1_A5, hrs_06_10_Rep2_A13, hrs_09_13_Rep1_A6, hrs_09_13_Rep2_A14, hrs_12_17_Rep1_A7, hrs_12_17_Rep2_A15, hrs_16_22_Rep1_A8, hrs_16_22_Rep2_A16. Cells were sorted on a Beckman Coulter MoFlo Astrios cell sorter (QMC, Nottingham) equipped with a 100 µm nozzle and operated in Purify precision mode using a 1–2 drop envelope to maximise the purity of collected fractions. Single-cell suspensions were maintained at 4 °C throughout sorting by enclosing the flow chamber in a water-cooled housing, thereby minimising heat-induced stress and preserving cell integrity. Sorted samples were collected into a two-way tube holder pre-chilled on ice. Gating strategies were defined using unstained and single-stained controls to exclude autofluorescent events. Broad gates were applied to capture both strongly and weakly fluorescent populations, reflecting the continuum of marker expression. SoxN⁺ and Elav⁺ cells—representing early neurogenic and post-mitotic neuronal cohorts, respectively—were collected together to ensure recovery of the full spectrum of developing neural cells. Data structure and format All files are provided in standard Flow Cytometry Standard (FCS 3.1) format. Keywords FACS, flow cytometry, neurogenesis, monoaminergic neurons, single-cell sorting, developmental biology数据集内容 本数据集包含对应以下样本的原始荧光激活细胞分选（fluorescence-activated cell sorting, FACS）数据：QC_Setup、hrs_00_03_Rep1_A1、hrs_00_03_Rep2_A9、hrs_02_05_Rep1_A2、hrs_02_05_Rep2_A10、hrs_04_07_Rep1_A3、hrs_04_07_Rep2_A11、hrs_05_08_Rep1_A4、hrs_05_08_Rep2_A12、hrs_06_10_Rep1_A5、hrs_06_10_Rep2_A13、hrs_09_13_Rep1_A6、hrs_09_13_Rep2_A14、hrs_12_17_Rep1_A7、hrs_12_17_Rep2_A15、hrs_16_22_Rep1_A8、hrs_16_22_Rep2_A16。 细胞分选在配备100 μm喷嘴的贝克曼库尔特MoFlo Astrios细胞分选仪（QMC，英国诺丁汉）上完成，该设备运行于纯化精准模式，采用1~2滴分选窗口以最大化收集组分的分选纯度。分选全程将流式室置于水冷外壳中，维持单细胞悬液处于4 ℃环境，以最小化热诱导应激、维持细胞完整性。分选后的样本被收集于预先置于冰上的双向试管架中。 门控策略通过未染色对照与单染色对照定义，以排除自发荧光事件。设置宽幅门控以同时捕获强荧光与弱荧光群体，反映标志物表达的连续分布特征。SoxN阳性（SoxN⁺）与Elav阳性（Elav⁺）细胞分别代表早期神经发生谱系及有丝分裂后神经元群，二者被一并收集以确保完整回收发育中的神经细胞全谱系。 数据结构与格式 所有文件均采用标准流式细胞术标准（Flow Cytometry Standard, FCS 3.1）格式存储。 关键词 荧光激活细胞分选，流式细胞术，神经发生，单胺能神经元，单细胞分选，发育生物学