Mechanomics RT-DC Set 05 - iPSCs

Entry is a part of <b>Mechanomics</b> collection (10.6084/m9.figshare.c.5399826) that contains materials supporting findings presented in the following manuscript:<i>Urbanska, M., Ge, Y.</i> et al.,<br><b><i>De novo identification of universal cell mechanics gene signatures</i></b>BiorXiv: https://doi.org/10.1101/2021.04.26.441418<i>;</i><i> </i><i>first published:</i><i> </i>27th April 2021<i>, last updated:</i><i> </i>4th Nov 2024<br>eLife: https://doi.org/10.7554/eLife.87930<i>; first published:</i><i> </i>23rd March 2023<i>, last updated:</i><i> </i>27th Dec 2024<br>This dataset contains mechanical measurements of murine induced pluripotent stem cells (iPSCs) of two different classes:<br>• transgene-dependent, fuzzy-colony forming - F-class<br>• transgene-independant, compact-colony forming - C-class.<br>These two cell types were first characterised by Tonge et al (<i>Nature, 2014</i>). The cell lines used in this study were derived in a previously published study by Urbanska et al (<i>Development, 2017</i>) via reprogramming of neural progenitor cells using a dox-induced expression of the OSKM reprogramming factors.Measurements were performed using real-time deformability cytometry (RT-DC).<b>_rtdc.zip</b> - contains data acquired during the measurments in '*.rtdc' format<br><b>_tsv.zip</b> - contains extended data in a '*.tsv' format<b>_glmm.zip</b> - contains result summaries of statistical analysis comparing Young's moduli of the different cell types using generalised linear mixed effects modelsTonge, P. D. <i>et al.</i> Divergent reprogramming routes lead to alternative stem-cell states. <i>Nature </i><b>516</b>, 192–197 (2014) https://doi.org/10.1038/nature14047Urbanska, M. <i>et al.</i> Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage. <i>Development </i><b>144</b>, 4313–4321 (2017) https://doi.org/10.1242/dev.155218<br>