WHONDRS 48 Hour Diel Cycling Study at the East Fork Poplar Creek in Tennessee, USA

This dataset is from a sampling campaign completed over the course of 48 hours to investigate the effects of wastewater treatment plant effluent induced water level variation on aqueous chemistry, dissolved organic matter chemistry, and metabolic potential of microbial communities at the East Fork Poplar Creek, TN, USA. Filtered surface water (SW) and pore water (PP) samples were collected every three hours in August 2018. This dataset is one of seven companion datasets that were part of the WHONDRS 48 Hour Diel Cycling Study of freshwater rivers. All seven datasets are available on ESS-DIVE; they can be found by searching for WHONDRS 48 Hour.This package contains a variety of chemical and hydrologic variables. All data types are provided in a standard format. Basic geochemistry analyses (e.g., non-purgeable organic carbon concentration, cations, anions, SpC, pH) were conducted, standardized photos were taken, surface water hydrographs were collated from existing instrumentation, and extensive metadata were captured. Aqueous chemistry variables include USGS codes in the first portion of the variable name (e.g., 00940_Cl_mg_per_L). These codes allow for interoperability with USGS water quality data. In addition, samples were delivered to the Environmental Molecular Sciences Laboratory (EMSL; https://www.pnnl.gov/environmental-molecular-sciences-laboratory) for high resolution characterization of dissolved organic matter via 12 Tesla Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). The data package contains instructions to process the FTICR data based on the user’s specific needs, and sample-level summary indices derived from the FTICR data will be added to the data package. Beyond the data in this data package, metagenomic data are being generated on associated microbial communities. When these data are available, the data package will be updated with information on accessing them. Please contact [e-mail] for more information. Please use the data package’s DOI to cite the data package. We ask that you email us at [e-mail] to let us know that you're using the data and acknowledge WHONDRS and the U.S. Department of Energy's Subsurface Biogeochemical Research program—which generously provides funding to WHONDRS—in your publications, proposals, presentations, etc. All data are free to be used for any purpose, such as for manuscripts, presentations, and grant proposals. Note that the included hydrograph has a separate DOI that should also be cited. There is no obligation to include WHONDRS members as co-authors.

本数据集源自一项耗时48小时的野外采样任务，旨在探究美国田纳西州东叉波普尔溪（East Fork Poplar Creek）处，污水处理厂出水引发的水位变化对水化学、溶解性有机质化学以及微生物群落代谢潜能的影响。2018年8月，研究团队每3小时采集一次过滤地表水（Surface Water, SW）与孔隙水（Pore Water, PP）样本。本数据集是WHONDRS淡水河流48小时昼夜循环研究配套的7个数据集之一，全部7个数据集均可在ESS-DIVE平台获取，搜索关键词“WHONDRS 48 Hour”即可定位到相关资源。

本数据包包含多类化学与水文变量，所有数据均采用标准格式存储。已完成基础地球化学分析，包括不可吹扫有机碳浓度、阳离子、阴离子、比电导率（Specific Conductance, SpC）、pH值等；拍摄了标准化照片；从现有监测仪器中整理得到地表水水文过程线，并采集了详尽的元数据。

水化学变量的名称前缀采用美国地质调查局（United States Geological Survey, USGS）代码（例如00940_Cl_mg_per_L），此类代码可实现与USGS水质数据的互操作性。此外，样本被送至环境分子科学实验室（Environmental Molecular Sciences Laboratory, EMSL；https://www.pnnl.gov/environmental-molecular-sciences-laboratory），通过12特斯拉傅里叶变换离子回旋共振质谱（Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, FTICR-MS）开展溶解性有机质的高分辨表征。本数据包包含针对FTICR数据的用户定制化处理指南，且由FTICR数据衍生的样本级汇总指标将后续添加至数据包中。

除本数据包内的现有数据外，相关微生物群落的宏基因组数据正在生成中。待相关数据正式可用后，本数据包将更新其获取方式说明。如需获取更多信息，请联系[e-mail]。请使用本数据包的数字对象标识符（Digital Object Identifier, DOI）进行引用。我们恳请您通过邮件[e-mail]告知您的使用意向，并在您的出版物、项目提案、学术汇报等材料中致谢WHONDRS以及为WHONDRS提供慷慨资助的美国能源部地下生物地球化学研究项目。

所有数据均可免费用于各类用途，包括学术论文撰写、汇报展示以及基金申请等。请注意，本数据包附带的地表水水文过程线拥有独立DOI，也需一并完成引用。无需强制将WHONDRS成员列为共同作者。