Labcode 1- HIV neutralization testing with pseudovirions
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下载链接：
https://doi.org/10.7910/DVN/SWC0QG
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资源简介：
This protocol includes a detailed protocol of HIV neutralizaion assay based on pseudovirus produced by co-transfection of pEnv with a lentiviral expression plasmid, pGJ3-luc, into HEK293T cells. The HEK293T cells was used in a neutralisation assay with monoclonal antibodies and sCD4. Target cells were 3T3 cells engineered to express CD4 and CCR5 or CXCR4, and the readout measured luciferase reporter gene expression.
创建时间：
2018-09-08
