Figure 7: Raw data, MATLAB scripts, and processed videos of lightsheet image stacks of algae cells

3D volume stacks of images of algae cells embedded in agarose. <i>C. vulgaris</i> and <i>D. quadricauda</i> cells were either treated with DCMU, left untreated as controls and a mixture of treated and untreated <i>C. vulgaris</i> cells. The fluorescence lifetime of the DCMU-treated cells was increased due DCMU blocking electron acceptor oxidation and thus reaction center quenching. There are three folders in the package:Dquadricauda_lightsheetCvulgaris_lightsheetCvulgaris_mixture_lightsheetEach folder contains a MATLAB script <b>makeFLIM.m</b>, which produces videos and graphs from the SPAD output data analyzed in TRI2. The calibration data from the SPADlinearization package (https://doi.org/10.18742/21325584) will be needed to run this script.This dataset is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"