Quartet of familiar males – 16p11.2 Del mouse strain – 2 WT + 2 Del/+ - M1
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We monitored the individual and social behaviours of each quartet of mice over three days and nights in the Live Mouse Tracker system (LMT, plugin 931; de Chaumont et al. 2019 Nat. Biomed. Engin.). This system tracks individually mice living in a group over several days and nights and extracts automatically the number, total duration and mean duration of more than thirty behavioural events describing the posture of the mouse, the types of social contacts, the dynamic social approach and escapes and complex social groupings (see de Chaumont et al. 2019 Nat. Biomed. Engin.). In this system, the four mice (10-14 weeks of age; 2 WT mice and 2 Del/+ mice) from the same housing cage (housed together from weaning on) were left undisturbed for 71 hours in a large transparent Plexiglas cage (50 x 50 x 40 cm), with fresh bedding, a house (width: 100 mm, depth: 75 mm, height: 40 mm) in red Plexiglas, 6 dental cotton rolls as well as food and water ad libitum. Light/dark cycle and temperature conditions were similar to those of the housing room (12/12h light/dark, lights on at 07:00 AM, 100 lux when the lights were on). Each recording session started between 03:00 and 04:00 PM. At the end of the session, mice were placed back in their home cage and the LMT setup was cleaned with soap water and dried with paper towels. The upload includes the sqlite database from LMT (processed).
本研究借助活体小鼠追踪系统(Live Mouse Tracker, LMT,插件版本931;de Chaumont等,2019,《自然·生物医学工程》),对每一组四只小鼠的个体行为与社交行为开展了为期三昼夜的监测。该系统可对群居饲养的小鼠进行多昼夜个体追踪,并自动提取三十余种行为事件的发生次数、总时长与平均时长,涵盖小鼠姿态、社交接触类型、动态社交趋近与逃逸行为,以及复杂社交群体互动等类别(详见de Chaumont等,2019,《自然·生物医学工程》)。 本次实验中,4只自断奶起即群居饲养的小鼠(周龄10~14周,含2只野生型(Wild Type, WT)小鼠与2只Del/+突变型小鼠)被置于尺寸为50×50×40 cm的透明有机玻璃饲养箱中,静置71小时不受干扰。饲养箱内配备新鲜垫料、1座红色有机玻璃躲避屋(宽100 mm、深75 mm、高40 mm)、6支牙科棉卷,以及自由采食的食物与饮水。 饲养环境的明暗周期与温度条件与原饲养笼保持一致:采用12/12小时的明暗循环,开灯时间为每日7:00,光照强度为100 lux。每次记录时段均于下午3:00至4:00之间启动。实验结束后,小鼠被放回原饲养笼,同时使用肥皂水清洁LMT系统装置,再以纸巾擦干。本上传内容包含经处理的LMT系统生成的SQLite数据库文件。



