De novo genome assembly of the Tobacco Hornworm moth (Manduca sexta)

<strong>We present the new reference genome for M sexta, JHU_Msex_v1.0, applying a combination of modern technologies in a de novo assembly to increase continuity, accuracy, and completeness. The assembly is 470 Mb and is ~25x more continuous than the original assembly, with scaffold N50 &gt;14 Mb. We annotated the assembly by lifting over existing annotations and supplementing with additional supporting RNA-based data for a total of 25,256 genes. The new reference assembly is accessible in annotated form for public use.</strong>我们发布了针对M sexta的全新参考基因组JHU_Msex_v1.0，通过整合多种现代技术开展从头组装（de novo assembly），以提升组装的连续性、准确性与完整性。本次组装的基因组总长度为470 Mb，连续性较原始组装提升约25倍，支架N50（scaffold N50）大于14 Mb。研究团队通过对现有注释进行注释迁移（lifting over）并补充额外的RNA测序支持数据，完成了该组装的注释工作，共获得25256个基因。该全新参考组装结果以注释版本形式对外开放，可供公众使用。