Homogeneous multifocal excitation for high-throughput super-resolution imaging - Expanded centriole particles

Zenodo2020-01-20 更新2026-04-07 收录

超分辨率成像

中心粒结构分析

数据链接：

https://zenodo.org/record/3613906 数据链接链接失效反馈

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资源简介：

Datasets containing the segmented expanded centriole particles. The prefix Hs is used to denote particles acquired in synchronized RPE-1 human cells. Otherwise particles were collected from expanded isolated centrioles from <em>Chlamydomoanas reinhardtii</em>. Resized datasets have uniform voxel size of 14x14x14 nm3 after expansion (56x56x56 nm3 before expansion). Non-resized datasets have 14x14x30 pixel size (56x56x120 nm3 before expansion). All files should be mirrored horizontally/vertically to account for the chirality inversion due to the imaging process The channels in different datasets are: Chlamy acetylated sample: C1: acetylated tubulin-Alexa488 C2: aTubulin-Alexa568 Chlamy MonoE sample C1: aTubulin-Alexa488 C2: GT335-Alexa568 Chlamy PolyE sample C1: PolyE-Alexa488 C2: aTubulin-Alexa568 Hs sample: C1: PolyE-Alexa488 C2: acetylated tubulin-Alexa586

提供机构：

Laboratory for Experimental Biophysics, Institute of Physics, Swiss Federal Institute of Technology Lausanne, Lausanne, Switzerland; Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland; Laboratory for Experimental Biophysics, Institute of Physics, Swiss Federal Institute of Technology Lausanne, Lausanne, SwitzerlandSwiss National Centre for Competence in Research in Chemical Biology, University of Geneva, Geneva, Switzerland; ICube, CNRS, University of Strasbourg, Illkirch, France; Swiss National Centre for Competence in Research in Chemical Biology, University of Geneva, Geneva, SwitzerlandSwiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology Lausanne, Lausanne, Switzerland

创建时间：

2020-01-20