Towards epigenetic regulation of triple-negative breast cancer via ligand-mediated nanoparticles - supplementary material

Aims: Develop and analyze triple-negative breast cancer targeted nanoparticles loaded with the

demethylating agent decitabine. Materials & methods: The polymers were synthesized by ring-opening

polymerization of D,l-lactide and formulated into nanoparticles via emulsion-evaporation method. The

nanoparticles were characterized by physicochemical analysis as well as in vitro using breast cancer

cell lineages. Results & conclusion: The targeted nanoparticles exhibited a hydrodynamic diameter of

75 ± 12 nm, zeta potential -6.3 ± 0.2 mV and spherical morphology, and displayed greater in vitro

accumulation into MDA-MB-231 (triple-negative breast cancer cell-line) compared with MCF7 and HB4A

cell lineages as verified by fluorescence confocal microscopy and significant demethylating effects via

ADAM33 screening by PCR.