JUNB is a critical AP1 component for SMAD2/3 binding after TGFß stimulation [RNA-seq]

We performed SMAD2/3 ChIP-seq analysis in MCF10A MII cells. To validate whether the changes in SMAD2/3 binding to the genome indeed resulted in changes in target gene expression, we performed RNA-seq transcriptome analysis after short and long periods of TGFß stimulation (0, 1.5h and 16h) in MII cells. In addition, we revealed that JUNB is a critical AP1 component for SMAD2/3 binding after TGFß stimulation. To assess the significance of JUNB for TGFß-SMAD-target genes on a genome-wide scale, we also performed RNA-seq transcriptome analysis in JUNB-knock-downed MII cells. Overall design: RNA-seq analysis in MCF10A MII cells with TGFß stimulation and/or JUNB knock down