Expression profiling of microRNA in the extracellular vesicles of rat's follicular fluid under high fat diet or high fat diet with acetic acid

This data presents the effect of acetic acid feeding on the obesity-induced alternation in miRNA profiles in the follicle fluid (FF). The follicle fluids were collected from Slc: SD rats which feed normal diet (ND), high fat diet (HFD) with water or high fat diet with water containing acetic acid (600uL/L)(HFD+V) for 7-11 weeks, and centrifugated to separate supernatant and cellular pellets followed by filtration (0.2um). The extracellular vehicles (EVs) were isolated from FFs using Exoquick. EVs RNA was extracted from the supernatant using a SeraMir Exosome RNA Amplification Kit (System Biosciences). RNA quality and concentration were examined using a Bioanalyzer (Agilent technologies, Palo Alto, CA, USA). cDNA library was constructed using an NEBNext Multiplex Small RNA-seq Library Prep Set for Illumina (New England BioLabs, Inc., Ipswich, MA) for RNA from FFs. Clusters were generated on a cBot (Illumina), and one lane of the multiplied samples was sequenced as 75 bp reads (single read) on the NextSeq 500 system (Illumina).