Longitudinal Healthspan Data

In order to assess healthspan, we performed a series of longitudinal, clinically-relevant healthspan measurements developed by Howlett and her colleagues. This frailty index (FI) consists of 31 phenotypes that are indicators of age-associated health deterioration, with each phenotype scored on a 0, 0.5 or 1 scale, based on its severity. All scorings were conducted in a blinded manner. The 31-metrics share many characteristics of human frailty indices and have been reported to progress similarly with aging in when compared with the well-established Rockwood frailty scale for humans. Measurements were repeated approximately every eight weeks, providing us with eight and seven sets of data respectively for male and female groups. The total frailty score, which is the average of 31 frailty phenotypes, was calculated for each animal at every time point .

为评估健康寿命，我们采用Howlett及其团队开发的一系列纵向、临床相关的健康寿命检测指标开展研究。该衰弱指数（frailty index, FI）包含31项表型（phenotype）指标，用于表征年龄相关性健康衰退，每项表型根据其严重程度以0、0.5或1的分值进行评分。所有评分均采用盲法实施。这31项指标与人类衰弱指数具备诸多共性，且已有研究表明，其随衰老的进展模式与成熟的人类罗克伍德（Rockwood）衰弱量表高度相似。检测每约8周重复一次，雄性组与雌性组分别获得8组和7组数据集。针对每个时间点的每只实验动物，计算其总衰弱评分——即31项衰弱表型指标的平均值。