Lucinid rRNA sequences for development of depletion kit
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To improve the sequencing depth of symbiont mRNA and thus facilitate the quantification of the expression of coding genes, both bacterial and host ribosomal RNA (rRNA) was depleted in accordance with the manufacturer's instructions using the general Pan-Bacteria riboPOOL kit (kit dp-P024-000026, siTOOLs Biotech), and a custom-designed host-specific riboPOOL kit (dp-K024-000089, siTOOLs Biotech), which was created using the various lucinid ribosomal RNA sequences available here.
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2024-11-13



