Arabidopsis thaliana strain:Col0 Transcriptome or Gene expression. Arabidopsis thaliana strain:Col0

The present project discerns the intricate expression profiles of genes within leaf RNA-Seq datasets of Arabidopsis thaliana subjected to salt stress (200 mM NaCl) at three distinct time points, e.g., 0h, 2h and 12h. The commencement of this investigation witnessed the initiation of surface-sterilized A. thaliana seeds, which were judiciously germinated and maintained for a duration spanning four days. Concomitant with this controlled growth regimen, diligent irrigation with deionized double-distilled water was administered on a daily basis, nurturing the seedlings to maturity over a span of three weeks. Thereafter, the crucible of salt stress imposition was initiated, commencing with a salinity exposure of 50 mM NaCl, sustained over a diurnal interval. This preliminary salinity challenge served as the harbinger for subsequent escalations in salinity stress, each increment marked by an addition of 75 mM NaCl, as per established protocols. The pivotal leaf tissues of 4-week-old plants, subjected to an ultimate salt concentration of 200 mM NaCl, were scrupulously harvested in triplicates at 2h and 12h intervals following the second incremental salinity escalation. In parallel, leaves originating from 4-week-old unstressed plants, herein termed "0h," were concomitantly procured and employed as a control cohort for comparative analysis. The RNA extraction process of the triplicates of each time point was undertaken and the pristine RNA samples were expedited to BGI, China, for subsequent next-generation sequencing endeavors.