RNAseq of partially paralyzed zebrafish embryos at 5 days post-fertilization compared to normal siblings

Sofa potato (sop) is a mutant zebrafish line, whose synaptic transmission at the neuromuscular junction is absent due to a point mutation in the δ subunit gene of the acetylcholine receptor (AChR), leading to paralysis of its skeletal muscles. To explore genetic changes in embryos caused by the lack of synaptic transmission, we performed RNA-seq analysis of normal siblings (sop+/?) and sop homozygous embryos (sop-/-) at 5 days post-fertilization.