A Cold-Active Glucanase from the Ruminal Bacterium Fibrobacter succinogenes S85

We previously characterized two endoglucanases, CelG and EGD, from the mesophilic ruminal anaerobe Fibrobacter succinogenes S85. Further comparative experiments have shown that CelG is a cold-active enzyme whose catalytic properties are superior to those of several other intensively studied cold-active enzymes. It has a lower temperature optimum, of 25°C, and retains about 70% of its maximum activity at 0°C, while EGD has a temperature optimum of 35°C and retains only about 18% of its maximal activity at 0°C. When assayed at 4°C, CelG exhibits a 33-fold-higher k(cat) value and a 73-fold-higher physiological efficiency (k(cat)/K(m)) than EGD. CelG has a low thermal stability, as indicated by the effect of temperature on its activity and secondary structure. The presence of small amino acids around the putative catalytic residues may add to the flexibility of the enzyme, thereby increasing its activity at cold temperatures. Its activity is modulated by sodium chloride, with an increase of over 1.8-fold at an ionic strength of 0.03. Possible explanations for the presence of a cold-active enzyme in a mesophile are that cold-active enzymes are more broadly distributed than previously expected, that lateral transfer of the gene from a psychrophile occurred, or that F. succinogenes originated from the marine environment.