Selection of genes for ChIP-seq

This is to select ORFs with no overlapping transcriptional unit (on the same strand) 275 nt downstream the PAS. Protein-coding genes that do not overlap with any other transcription unit (on the opposite strand) in a region from 250 nt upstream to 500 nt downstream of the PAS were selected for the analysis.本研究旨在筛选多聚腺苷酸化位点（Polyadenylation Site, PAS）下游275 nt范围内，无与同链转录单元（transcriptional unit）重叠的开放阅读框（Open Reading Frame, ORFs）。本次分析选取的蛋白编码基因需满足：在PAS上游250 nt至下游500 nt的区域内，未与任何反链转录单元发生重叠。