Mathematical model of eATP regulation in non-polarized Caco-2 cells.

Numerical values of constants were normalized by the protein cell mass in the experiments (Mcell), measured by the Bradford method (section 4.7 in the manuscript). Parameter fitting and simulations were performed by selecting the average cell mass in the experiments (Mcell = 0.2 mg). JL and JNL represent the lytic and non-lytic iATP release respectively upon an osmotic shock. The value of these terms was 0 before shock application. Jleakage represents a constant and small iATP release observed in the absence of any stimulus. The parameter values obtained from the model fitting are expressed as the best value ± standard deviation.
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