Comparison of Pectobacterium carotovorum RC5297 transcription profiles depending on presence or absence of the PcaRCI RM system

This project aimed to investigate the transcriptomic differences between a Pectobacterium carotovorum wild-type strain (RC5297) and a derivative in which a restriction-modification system, termed PcaRCI, is knocked out. The aim was to identify genes whose expression might be regulated through methylation by the methyltransferase of the RM system. Overall design: Examination of two different bacterial strains (P. carotovorum RC5297 wild type and P. carotovorum RC5297 PcaRCI RM knockout) with five replicates each (ten samples in total)