Exploring the role of RFX6 in pancreatic islet development using iPSCs

Project manager(s): Essam M. Abdelalim (Sidra Medicine, Qatar) Wild type iPSCs (WT-iPSCs) generated and fully characterized in our laboratory were used for RFX6 knockout iPSCs (RFX6 KO iPSCs) generation. WT-iPSCs and RFX6 KO iPSCs were differentiated into pancreatic islet cells to uncover RFX6’s role during pancreatic islet development. The dataset represents RNA-seq data generated from pancreatic progenitors (PPs) and endocrine progenitors (EPs) derived from WT-iPSCs and RFX6 KO iPSCs. The file name is: Sample name _overall sample number_read direction_001 where: - PPs-WT-Rep1: pancreatic progenitors (PPs) derived from WT-iPSCs, replicate 1. - PPs-WT-Rep2: pancreatic progenitors (PPs) derived from WT-iPSCs, replicate 2. - PPs-WT-Rep3: pancreatic progenitors (PPs) derived from WT-iPSCs, replicate 3. - PPs-KO-Rep1: pancreatic progenitors (PPs) derived from RFX6 KO iPSCs, replicate 1. - PPs-KO-Rep2: pancreatic progenitors (PPs) derived from RFX6 KO iPSCs, replicate 2. - PPs-KO-Rep3: pancreatic progenitors (PPs) derived from RFX6 KO iPSCs, replicate 3. - EPs-WT-Rep1: endocrine progenitors (EPs) derived from WT-iPSCs, replicate 1. - EPs-WT-Rep2: endocrine progenitors (EPs) derived from WT-iPSCs, replicate 2. - PPs-KO-Rep1: endocrine progenitors (EPs) derived from RFX6 KO iPSCs, replicate 1. - PPs-KO-Rep2: endocrine progenitors (EPs) derived from RFX6 KO iPSCs, replicate 2. - Read direction: R1 (Forward), R2 (Reverse).