The MIP (MIPFOC01) was mixed with different amounts of Foc genomic DNA (160 ng, 80 ng, 40 ng, 20 ng, 10 ng, 5 ng, 2.5 ng and 1.25 ng) in triplicates and analyzed in a MIP assay. The PCR amplicon was e
Column ‘ddPCR Copies (Reference)’ shows ddPCR validation of controls for cell lines containing mutations of interest. Source DNA was isolated from named cell lines or commercially sourced human placen
The DNA source (brook trout tissue, Plant Creek and Miller Creek filter extractions, and a mixed brook/bull trout DNA solution diluted 1∶10), estimated concentration, number of replicates, and proport
1LOD, limit of detection; LOQ, limit of quantification; values are calculated assuming maximal injection volume;2The UV signal for was correlated with fluoresence for enhanced sensitivity.
