GPDB

This FAIRsharing record describes: GlycoProtDB is a glycoprotein database providing information of Asn (N)-glycosylated proteins and their glycosylated site(s), which were constructed by employing a bottom-up strategy using actual glycopeptide sequences identified by LC/MS-based glycoproteomic technologies. The database is searchable using gene ID, gene name, and its description (protein name) as query. Each data sheet of glycproteins is based on a single amino acid sequence in Wormpep database for C.elegans and NCBI Refseq database for mouse. The sheet presents actually detected N-glycosylation site(s) which are displayed each capturing methods of glycopeptide subset, e.g., lectins Concanavalin A, wheat germ agglutinin (WGA), or HILIC (hydrophilic interaction chromatography), as well as potential N-glycosylation sites (NX[STC], X?P). Protein sequences, which have common glycopeptide sequence(s), are linked each other.

本FAIRsharing记录所描述的GlycoProtDB是一款糖蛋白质数据库，收录天冬酰胺（Asn）位点N-糖基化蛋白质及其糖基化位点的相关信息。该数据库采用自下而上策略构建，数据来源于基于液相色谱-质谱（LC/MS）的糖蛋白质组学技术所鉴定得到的真实糖肽序列。用户可通过基因ID、基因名称及蛋白质描述信息（蛋白质名称）作为查询词对该数据库进行检索。针对秀丽隐杆线虫（C.elegans），其糖蛋白质数据表基于Wormpep数据库中的单条氨基酸序列；针对小鼠，则基于NCBI参考序列（RefSeq）数据库中的单条氨基酸序列。每张数据表均展示了实际检测到的N-糖基化位点，这些位点会根据糖肽子集的富集捕获方法进行分类展示，例如伴刀豆球蛋白A（Concanavalin A）、小麦胚芽凝集素（WGA）或亲水相互作用色谱（HILIC），同时还会标注潜在N-糖基化位点（NX[STC]，且X不为脯氨酸）。拥有共同糖肽序列的蛋白质序列之间会建立关联链接。