The response to a set of potential inducers for the original templates and the 5.3p3c19 clone grown in M9 22 h after induction.

The response is expressed as the % increase in fluorescence when 500 µM of the potential inducer is added compared to the control where only solvent (DMSO) is added. The mean fluorescence was measured for a cell population of 10 000 cells for each sample with the standard error based on at least three independent experiments shown. The basal level of fluorescence (Basal F) is compared to that of WT DntR grown in M9 at the same signal amplification and is measured as % increase in fluorescence for mutant grown with DMSO/fluorescence for wt grown with DMSO. (Wt grown in LB gives a 100%(±30) increase in fluorescence compared to wt grown in M9). SAL: salicylate, DNT: 2,4-dinitrotoluene, 2-NT: 2-nitrotoluene, 2-NB:2-nitrobenzoate, 4-NT: 4-nitrotoluene, 4-NB: 4-nitrobenzoate, BEN: benzoate.