Characterization of the adaptive transcriptional response triggered by the cell wall interfering compound Poacic acid in yeast and its effect on crosslinking enzymes.

We analyzed the genome-wide expression profile of the wild type strain in response to poacid acid (1 hour of treatment). In addition, we did transcription profiling on the effect of slt2 (MAPK of Cell wall intregity pathway) deletionin genes involved in poacid acid response Overall design: For poacid acid treatment, three biological samples for wild type strain and three biological samples for slt2 mutant were analyzed. Two RNA-seq experiments were carried out for each biological sample: 1. absence of the drug 2. presence of 100 µg/ml of poacid acid for one hour