Overcoming sample limitations to enable time-resolved characterisation of a wider range of protein dynamics
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https://doi.esrf.fr/10.15151/ESRF-ES-2237124755
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资源简介：
Here we propose method development at the time-resolved X-ray solution scattering (TR-XSS) beamline ID09 to enable characterization of a wider range of protein targets. Our previous studies have provided critical insights into structural intermediates of eukaryotic calcium transporter SERCA1a, cooperative protein dynamics in adenylate kinase, and the rate-limiting step in bacterial calcium transport. However, the need for large sample volumes in pump-based delivery systems have limited the target proteins and also caged compounds. To overcome these limitations, we propose the use of a fixed-target microchamber chip, which will dramatically reduce demands on both the protein and caged compound. Successful implementation will enable the study of challenging protein targets such as human G-protein coupled receptors (GPCRs) and ligand-gated ion channels (LGICs), which are critical to human physiology and disease intervention.
提供机构：
Slovak Academy of Sciences, Centre of Experimental Medicine, v.v.i., Dúbravská cesta 9 Bratislava, 84104, Bratislava, SLOVAKIA; University of Michigan, Center for Arrhythmia Research/Internal Med, 2800 Plymouth Rd, 48109, Ann arbor, USA; Umea University, Department of Chemistry, Linnaeus Väg 10, 90187, Umea, SWEDEN; Umea University - KBC, Department of Chemistry, Linnaeus Vag 6, 90 187 Umea, Sweden; Lund University, MAX IV Laboratory, Fotongatan 2, 22484, Lund, SWEDEN
创建时间：
2028-01-01
