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Raw Nanopore sequencing data of CD-pore-seq V1: TelN PromethION SRICO_PromeR1 10

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Zenodo2026-05-11 更新2026-05-26 收录
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=Raw Nanopore sequencing data of CD-pore-seq V1= ==Author==Zicheng Yu1, Zihao Chen1, Thomas C. Williams1,2, Craig J. Anderson1,3,4, Andrew P. Jackson1,*, Martin A.M. Reijns1,*, Martin S. Taylor1,*1 MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh, UK.2 Child Life and Health, University of Edinburgh, UK.3 German Cancer Research Center, Heidelberg, Germany4 Department of Translational Oncology, German Cancer Research Center, Munich, Germany. * Correspondence should be addressed to andrew.jackson@ed.ac.uk, martin.reijns@ed.ac.uk and martin.taylor@ed.ac.uk ==Citation=='Generalised training for DNA modification detection by nanopore sequencing' ==Description==Nanopore sequencing data of libraries to train machine learning models to predict single DNA-embedded ribonucleotides.Ligation kit: LSK-114 (all except for ygDNA) or SQK-NBD114.96 (ygDNA)Flow-cell version: R10.4.1Sequencing device: MinION or PromethION (./TelN/PromethION only) ==TelN==* Sequencing the TelN library (high-complexity randomers surrounding single DNA-embedded ribonucleotides) on a MinION flow-cell (./MinION) or two PromethION flow-cells (PromethION), prepared by CD-pore-seq (with the second strand synthesised by the primer containing TelN recognition sequence, cut and covalently linked by TelN digestion).

=CD-pore-seq V1原始纳米孔测序(Nanopore sequencing)数据= ==作者==Zicheng Yu¹, Zihao Chen¹, Thomas C. Williams¹,², Craig J. Anderson¹,³,⁴, Andrew P. Jackson¹,*, Martin A.M. Reijns¹,*, Martin S. Taylor¹,*¹ 英国爱丁堡大学遗传学与癌症研究所MRC人类遗传学研究室;² 英国爱丁堡大学儿童生命与健康系;³ 德国海德堡德国癌症研究中心;⁴ 德国慕尼黑德国癌症研究中心转化肿瘤学系。 * 通讯作者邮箱:andrew.jackson@ed.ac.uk、martin.reijns@ed.ac.uk、martin.taylor@ed.ac.uk ==引用==《纳米孔测序检测DNA修饰的通用训练方法》(原英文标题:"Generalised training for DNA modification detection by nanopore sequencing") ==数据集描述==本数据集为用于训练机器学习模型以预测单个DNA嵌入核糖核苷酸的文库的纳米孔测序数据。连接试剂盒:LSK-114(ygDNA样本除外)或SQK-NBD114.96(仅用于ygDNA样本);流动槽版本:R10.4.1;测序设备:MinION或PromethION(仅./TelN/PromethION组适用)。 ==TelN==* TelN文库由CD-pore-seq技术制备,该文库包含环绕单个DNA嵌入核糖核苷酸的高复杂度随机序列,可在MinION测序流动槽(./MinION组)或两台PromethION测序流动槽(PromethION组)上进行测序;其第二条链通过携带TelN识别序列的引物合成,经TelN酶切后完成共价连接。

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2026-03-12
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