Adaptability of Cobetia marina to growth at different temperatures

The bacteria Cobetia marina is globally distributed in different oceanic ecosystems and is able to grow in a wide range of temperatures from 0°C to 41°C, with an optimal growth temperature close to 35°C. Its remarkable characteristics include its ability to form biofilm, produce biosurfactants, and use petroleum-derived hydrocarbons as energy and carbon source. In this context, Cobetia marina is a model for biofilm production and the production of diverse biomolecules of relevance for human and animal health, as well as for environmental protection and recovery. This dataset shows the differential expression of genes and gene regulatory circuits that explain the adaptation of Cobetia marina to different temperatures, serving as a model of evolution in response to selection pressures such as temperature changes in the marine environment. In this study, Cobetia marina was grown at 16°C, 35°C, 38°C, and 41°C to establish differential gene expression using high-throughput RNA sequencing. Overall design: To investigate how temperatures trigger different gene regulatory circuits, mediating the differential adaptation of Cobetia marina strain MM1IDA2H-1, this bacterium was grown at 16°C, 35°C, 38°C, and 41°C. All experiments were carried out using 96-wells microplates. For each experimental condition, 95 cultures were grown until mid-exponential phase, in triplicates. Cells from each replicate were harvested and pooled for RNA extraction. Total RNA was sequenced using an Illumina pair-end protocol (2x 150 bp). Gene expression was quantified, and differential expressions were calculated considering 35 ºC as control condition.