Antibiotic resistance, production of extended-spectrum beta-lactamases (ESBLs), carbapenemases, and molecular epidemiology of Klebsiella pneumoniae

Characterisation and investigation of the molecular epidemiology and beta-lactam resistance of invasive K. pneumoniae isolates from a private laboratory in Gauteng, South Africa. This included antibiotic susceptibility profiles for isolates, confirmation of phenotypic production of ESBL, AmpC and carbapenemase, and detection of specific resistance genes. Genotyping of isolates was conducted using pulsed field gel electrophoresis and subsequently a dendrogram was constructed using the  pulsed field gel electrophoresis (PFGE) profiles of each isolate to identify pulsotypes. Genomic investigation was conducted on selected representative isolates using Next Generation Sequencing. Bioinformatics analysis was conducted on the NGS data.

对南非豪登省一家私人实验室中分离的侵袭性肺炎克雷伯菌菌株的分子流行病学及β-内酰胺类抗生素耐药性进行表征与研究。该研究包括对分离菌株的抗生素敏感性谱分析，对ESBL、AmpC和碳青霉烯酶表型产生的确认，以及特定耐药基因的检测。采用脉冲场凝胶电泳技术对菌株进行基因分型，并根据每个菌株的脉冲场凝胶电泳（PFGE）图谱构建树状图以识别脉冲型。对选定的代表性菌株采用下一代测序技术进行基因组研究。对下一代测序（NGS）数据进行了生物信息学分析。