UMAP channel value files

These files are channel value exports from FlowJo representing N=3 uninfected and N=3 Cal/07/09 infected NHBE cultures differentiated for 3 weeks at air-liquid interface in Pneumacult media. To create these files, populations were manually gated in FlowJo to the major cellular populations (see manuscript for staining and gating details). For each defined population, a channel value csv file was exported and a column was added listing the major population from which the file came. Once all 9 populations for a single sample were exported, the csv files were concatenated to create a single file. These files are used as input for making UMAPs in Figure 1B and 2A of the manuscript.

本数据集为FlowJo软件导出的通道值文件，对应3例未感染及3例感染Cal/07/09毒株的正常人类支气管上皮细胞（Normal Human Bronchial Epithelial, NHBE）培养物。该培养物采用气液界面培养体系，于Pneumacult培养基中分化3周。
文件制备流程如下：在FlowJo中通过手动门控将细胞分选为主要细胞亚群（染色方案与门控细节详见手稿正文）；为每个已定义的细胞亚群导出通道值CSV文件，并新增一列以标注该文件所属的主要细胞群；待单个样本的全部9个细胞亚群均完成导出后，将所有CSV文件合并为单个整合文件。
本数据集可作为输入文件，用于绘制本文手稿中图1B与图2A的均匀流形近似与投影（Uniform Manifold Approximation and Projection, UMAP）可视化结果。