The stout razor clam mesocosm experiment with microbial community composition data and PAH dioxygenase gene copy number data

The dataset provides bacterial 16S rDNA sequencing data to show the composition changes in bacterial communities resulting from the effect of bioturbation and oil exposure. Another part of this dataset illustrates the effect of bioturbation and oil exposure on a growth increase of bacterial population, which could degrade PAH more efficiently. To characterize that a quantitative PCR assay was developed to calculate the gene copy number for the key PAH degrading enzyme (PAH ring hydroxylating dioxygenase). The number of genes equals the number of cell possessing this enzyme assuming that there is one gene copy per cell. This dataset provides information on microbial community composition in the FASTA file titled “RazorClam2014.fasta” and the gene-copy number in the spreadsheet titled “Dataset-RazorClam2014”.

本数据集提供细菌16S rDNA测序数据，用以展示生物扰动与石油暴露共同作用下细菌群落的组成变化。本数据集的另一部分则阐明了生物扰动与石油暴露对可高效降解多环芳烃（PAH）的细菌种群生长增殖的影响。为表征相关降解特性，研究开发了定量PCR检测方法，用于计算关键多环芳烃降解酶（PAH环羟化双加氧酶，PAH ring hydroxylating dioxygenase）的基因拷贝数。本数据集假设每个细胞仅携带一个该酶的基因拷贝，因此基因拷贝数即等同于携带该基因的细胞数量。本数据集包含两类信息：其一为存储于名为"RazorClam2014.fasta"的FASTA格式文件中的微生物群落组成数据；其二为存储于名为"Dataset-RazorClam2014"的电子表格中的基因拷贝数信息。