Dataset 1-Flow Cytometer Sensitivity: A Quadratic Model

Q and B calculation. Dataset 1 was provided by the NIH Vaccine Research Center and involved calibrator beads (Thermo Scientific Duke Standards Corporation) run through an LSRII cytome- ter. MFIs are associated to duke_Beads_xxxV.fcs MESFs are associated to SC-...
Notes:
Data analysis was conducted using R/BioConductor. We used R’s implementation for K-means clustering to enable the automatic gating and the statistics extraction. The R functions for regression analysis lm was used to fit linear and quadratic regression models. The R function Poly was used to evaluate orthogonal polynomials involved in solving the resulting linear system of equations. We developed a freely available open source R/BioConductor package flowQB to automate the main steps for Q and B calculation, available through CRAN/Bioconductor. It includes a vignette that provides a task-oriented description of its functionality and an executable example that is intended to be used interactively. Thanks to everybody who provided FCS files for this collection! Please email Ryan Brinkman ([e-mail]) if you have any questions. MESF calibration used p=357217/7102 for voltage 600V, Calibrator Mean =7102 and Calibrator Standard Value =357217.