Summary of two-cell embryo and live offspring efficiencies of current and prior studies evaluating various techniques to improve penetration of the zona pellucida (ZP) of vitrified-warmed (V-W) mouse oocytes by spermatozoa.

Laser-assisted IVF is abbreviated as LAIVF, and vitrification is abbreviated as vit.aKunming mice are outbred, and C57BL/6 mice are inbred.bPercent two-cell embryos was calculated as # two-cell embryos/# total cells post-recovery and prior to treatment *100; data presented differently in the original manuscript were reconfigured to match the format of data presentation in the current study.cPercent live offspring was calculated as # live offspring/# two-cell embryos transferred to all recipients (pregnant and nonpregnant) *100; data presented differently in the original manuscript were reconfigured to match the format of data presentation in the current study.NA indicates data not available.激光辅助体外受精（Laser-assisted IVF）缩写为LAIVF，玻璃化冷冻（vitrification）缩写为vit.。a. 昆明小鼠为远交系小鼠，C57BL/6小鼠为近交系小鼠。b. 双细胞胚胎占比计算公式为：（回收后、处理前的双细胞胚胎数量/总细胞数量）×100；原稿中数据呈现格式与本研究存在差异，已重新调整以匹配本研究的数据呈现规范。c. 活产子代占比计算公式为：（移植至所有受体（含妊娠与非妊娠受体）的双细胞胚胎所对应的活产子代数量/移植的双细胞胚胎总数）×100；原稿中数据呈现格式与本研究存在差异，已重新调整以匹配本研究的数据呈现规范。NA表示数据不可用。