Analysis of purified wild type and mutant adenovirus particles by SILAC based quantitative proteomics.

Data from ProteomeXchange, PXD ID: PXD001120. File: 10_2.mzml. Published as part of J Gen Virol. 2014 Aug 5 . From the Abstract: {{i}} We used stable isotope labelling of amino acids in cell culture and high throughput quantitative mass spectrometry to analyse the protein composition of highly purified wild type adenoviruses, mutant adenoviruses lacking an internal protein component (protein V) and recombinant adenoviruses of the type commonly used in gene therapy including one virus which had been used in a clinical trail. We found that the viral protein abundance and composition was consistent across all types of virus examined except for the virus lacking protein V which also had reduced amounts of another viral core protein, protein VII. In all the samples analysed we found no evidence of consistent packaging or contamination with cellular proteins ... {{/i}}