Population genetic consequences of habitat fragmentation in ectomycorrhizal fungi with different dispersal mechanisms: Implications from ice-aged relict forests across the Japanese archipelago

Recent human activities have caused extensive forest fragmentation, which can profoundly affect the population genetic structure of soil microbes, as has been well documented for plants and animals. However, such information remains scarce for microbes, even for ectomycorrhizal (ECM) fungi, which play key roles in the growth and survival of dominant forest trees. To investigate the long-term effects of forest fragmentation on the population genetic structure of ECM fungi, we examined ice-age relict forests of Pinus pumila across nine alpine sites that have been isolated for more than 10,000 years. We focused on two phylogenetically related ECM fungi, Rhizopogon alpinus and Suillus spraguei, whose spores are dispersed primarily by animals and wind, respectively. The genetic structure of these fungi and their host trees was assessed via 28 microsatellite markers, 14 of which were newly developed in this study. R. alpinus, an animal-dispersed species, exhibited substantially greater geneti..., , # Data from: Population genetic consequences of habitat fragmentation in ectomycorrhizal fungi with different dispersal mechanisms: Implications from ice-aged relict forests across the Japanese archipelago

## Table.genotype_microsatellite_144_Rhizopogon_alpinus.xlsx

Diploid genotypes of 144 *Rhizopogon alpinus* individuals (rows) for seven microsatellite markers (columns). First column: sample ID. Second column: Population ID. Third column onward: microsatellite alleles.

## Table.genotype_microsatellite_185_Suillus_spraguei.xlsx

Diploid genotypes of 185 *Suillus_spraguei* individuals (rows) for eight microsatellite markers (columns). First column: sample ID. Second column: Population ID. Third column onward: microsatellite alleles.

## Table.genotype_microsatellite_262_Pinus_pumila.xlsx

Diploid genotypes of 262 *Pinus_pumila* individuals (rows) for 13 microsatellite markers (columns). First column: sample ID. Second column: Population ID. Third column onward: microsatellite alleles...,

近年来人类活动引发了广泛的森林生境片段化，该过程可深刻影响土壤微生物的种群遗传结构——这一效应在动植物类群中已有充分研究佐证。然而针对微生物的相关研究仍较为匮乏，即便是在优势乔木生长与存活中发挥关键作用的外生菌根（ectomycorrhizal, ECM）真菌也不例外。为探究森林片段化对外生菌根真菌种群遗传结构的长期影响，本研究选取日本列岛9处已隔离超1万年的高山冰期孑遗偃松（Pinus pumila）林作为研究对象。本研究聚焦于两个系统发育关系相近的外生菌根真菌：高山根桩菇（Rhizopogon alpinus）与斯普拉格乳牛肝菌（Suillus spraguei），二者的孢子传播方式分别以动物和风力为主。本研究通过28个微卫星标记对两种真菌及其宿主树木的种群遗传结构进行评估，其中14个标记为本研究新开发。高山根桩菇作为动物传播的物种，展现出显著更高的遗传……

# 数据来源：不同传播机制外生菌根真菌的生境片段化种群遗传效应——基于日本列岛冰期孑遗森林的研究

## 表.genotype_microsatellite_144_Rhizopogon_alpinus.xlsx
144株高山根桩菇（Rhizopogon alpinus）个体的二倍体基因型（行），对应7个微卫星标记（列）。第一列为样本ID，第二列为种群ID，第三列及之后为微卫星等位基因。

## 表.genotype_microsatellite_185_Suillus_spraguei.xlsx
185株斯普拉格乳牛肝菌（Suillus spraguei）个体的二倍体基因型（行），对应8个微卫星标记（列）。第一列为样本ID，第二列为种群ID，第三列及之后为微卫星等位基因。

## 表.genotype_microsatellite_262_Pinus_pumila.xlsx
262株偃松（Pinus pumila）个体的二倍体基因型（行），对应13个微卫星标记（列）。第一列为样本ID，第二列为种群ID，第三列及之后为微卫星等位基因……