ChIP-seq analysis of H3K9ac and H4K16ac

We report the application of H3K9ac- and H4K16ac-based sequencing technology for high-throughput profiling of histone modifications in human granulosa KGN cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of KGN cells. We find that H3K9ac effectively discriminates genes that are influenced by butyrate treatment. H4K16ac described less influence of genes by butyrate treatment. Finally, we show that butyrate could mediate the H3K9ac to effectively influence genes that related to biological functions ang signalings. This study provides a framework for the application of comprehensive gene profile towards H3K9ac regulation by butyrate in human granulosa KGN cells. Examination of 2 different histone modifications in KGN cells by butyrate treatment.