DataSheet8_Matrine, a potential c-Myc inhibitor, suppresses ribosome biogenesis and nucleotide metabolism in myeloid leukemia.xlsx

Previous studies have shown that matrine, a natural compound extracted from the herb Sophora flavescens, has a good anti-leukemia effect, but its key target and mechanism remains unclear. Here, we found that only c-Myc could respond rapidly to matrine treatment in three myeloid leukemia cell lines, and matrine inhibited both transcription and translation of c-Myc. Ribosome biogenesis and nucleotide metabolism, the key downstream of c-Myc, were significantly suppressed after matrine treatment. Therefore, our results confirmed that matrine is a special c-Myc inhibitor which suppresses ribosome biogenesis and nucleotide metabolism by inhibiting c-Myc in myeloid leukemia. This study provides scientific basis for the development of matrine derivatives to c-Myc-driven cancers.既往研究表明，从植物 Sophora flavescens 中提取的天然化合物——土茯苓苷，具有显著的抗白血病作用，但其关键靶点与作用机制尚不明确。本研究发现，在三种髓系白血病细胞系中，仅有 c-Myc 对土茯苓苷治疗反应迅速，且土茯苓苷能够抑制 c-Myc 的转录和翻译。经过土茯苓苷处理后，c-Myc 的关键下游事件——核糖体生物合成与核苷酸代谢显著受到抑制。因此，我们的研究证实了土茯苓苷是一种特殊的 c-Myc 抑制剂，它通过抑制髓系白血病中的 c-Myc 来抑制核糖体生物合成与核苷酸代谢。本研究为开发针对 c-Myc 驱动的癌症的土茯苓苷衍生物提供了科学依据。